Recombinant Human 2,3-Bisphosphoglycerate Mutase, His-tagged

• Cat.No.: BPGM-667H
• Product Overview: Recombinant human BPGM, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 31kDa (267aa).

Specification

• Cat. No.: BPGM-667H
• Description: Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic synthesis of 2,3-Bisphosphoglycerate (2,3-BPG) from 1,3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active.
• Source: E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EF D LVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNERHYGALIGLNR EQMAL NHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSE SLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLP TGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: BPGM 2,3-bisphosphoglycerate mutase [ Homo sapiens ]
• Synonyms: 2,3-bisphosphoglycerate mutase; BPGM; bisphosphoglycerate mutase; BPG-dependent PGAM; 2,3-bisphosphoglycerate synthase; erythrocyte 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte; OTTHUMP00000207869; EC 5.4.2.4,EC 5.4.2.1,EC 3.1.3.13
• Gene ID: 669
• mRNA Refseq: NM_001724
• Protein Refseq: NP_001715
• MIM: 222800
• UniProt ID: P07738
• Chromosome Location: 7q31-q34
• Pathway: Glycolysis / Gluconeogenesis; Metabolic pathways
• Function: 2,3-bisphospho-D-glycerate 2-phosphohydrolase activity; bisphosphoglycerate mutase activity; hydrolase activity; isomerase activity; phosphoglycerate mutase activity