Recombinant Horse LEP protein, His & T7-tagged

• Cat.No.: LEP-673H
• Product Overview: Recombinant Horse LEP aa. (Ile15~Ala141 (Accession # Q9TU09)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Horse
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ile15~Ala141
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O

Gene Information

• Gene Name: LEP leptin [ Equus caballus (horse) ]
• Official Symbol: LEP
• Synonyms: obesity factor; leptin
• Gene ID: 100034042
• mRNA Refseq: NM_001163980.1
• Protein Refseq: NP_001157452.1
• UniProt ID: Q9TU09