Active Recombinant Ovine Leptin Antagonist Triple Mutant, PEG-tagged

• Cat.No.: LEP-264O
• Product Overview: Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 35.6 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. However due to enlarged hydrodymanic volume it runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200kDa protein. Leptin Antagonist Triple Mutant Ovine Recombinant Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques.

Specification

• Species: Ovine
• Source: Ovine
• Tag: Non
• Form: The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Purity: Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Bio-activity: Pegylated triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist like in mouse leptin antagonists), resulting mainly from increased food intake.
• Physical Appearance: White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored C. Upon reconstitution atμdesiccated below -18 > 0.1 Lep-tA mutant mg/ml and C or evenμup to 2mM and filter sterilization Leptin mutant can be stored at 4 room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: LEP leptin [ Ovis aries ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; ob; leptin (obesity homolog, mouse)
• Gene ID: 443534
• UniProt ID: Q28603