Description : |
Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. |
Source : |
E. coli |
Species : |
Human |
Form : |
Liquid |
Bio-activity : |
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or euqal to 0.005 ng/ml. |
Molecular Mass : |
17 kDa |
AA Sequence : |
MAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
Endotoxin : |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
Purity : |
> 95% by SDS-PAGE |
Storage : |
Can be stored at 4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
1 mg/mL (determined by BCA assay) |
Storage Buffer : |
PBS pH7.4 |
Tag : |
Non |
Protein length : |
117-269 a.a. |