Active Recombinant Human IL1B (117-269aa)

• Cat.No.: IL1B-027E
• Product Overview: Recombinant Human IL1B (Ala117 ­ Ser269) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: IL­1 is a name that designates two pleiotropic cytokines, IL­1μ (IL­1F1) and IL­1μ (IL­1F2), which are the products of distinct genes. IL­1μ and IL­1μ are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL­1μ and same receptor and exert identical biological effects. IL­1 RI binds directly to IL­1μ or IL­1μ and then associates with IL­1 R accessory protein (IL­1 R3/IL­1 R AcP) to form a high­affinity receptor complex that is competent for signal transduction. IL­1 RII has high affinity for IL­1μ but functions as adecoy receptor and negative regulator of IL­1μ activity. IL­1ra functions as a competitive antagonist by preventing IL­1μ and IL­1μ from interacting with IL­1 RI. The human IL­1μ cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL­1μ­converting enzyme (Caspase­1/ICE) to generate the active cytokine.
• N­terminal Sequence Analysis: Ala117
• Molecular Mass: 17 kDa
• Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically < 12 pg/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 3 month, 2 to 8 °C under sterile conditions after reconstitution.
• Unitprot ID: P01584

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Homo sapiens ]
• Official Symbol: IL1B
• Synonyms: IL1B; interleukin 1, beta; IL-1; IL1F2; IL1-BETA; interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• Chromosome Location: 2q14
• Pathway: Alzheimer"s disease; Apoptosis; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; MAPK signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; negative regulation of insulin receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-1 receptor binding; protein domain specific binding