Recombinant Hepatitis C virus (HCV) (serotype 1b, isolate HJ4) E2 protein(Glu384-Glu661), His-tagged

• Cat.No.: E2-3380H
• Product Overview: Recombinant Hepatitis C virus (HCV)(serotype 1b,isolate HC-J4E) E (AAC15723.1)(Glu384-Glu661) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: HCV
• Source: HEK293
• Tag: His
• Protein Length: Glu384-Glu661
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Hepatitis C virus (HCV)(serotype 1b,isolate HC-J4E) E consists of 289 amino acids and predicts a molecular mass of 32.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.