Recombinant HCV E2 Protein, His-tagged

• Cat.No.: E2-735V
• Product Overview: Recombinant Hepatitis C virus (serotype 1a, isolate H77) E2 protein (NP_751921.1) (Glu1-Glu278) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: HCV
• Source: HEK293
• Tag: His
• ProteinLength: 1-278 a.a.
• Predicted N Terminal: Glu
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant virus E2 consists 289 amino acids and predicts a molecular mass of 32 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.