Recombinant HCoV-OC43 Spike (S1+S2 ECD) protein(Met1-Pro1304), His-tagged

• Cat.No.: Spike-1226V
• Product Overview: Recombinant Human coronavirus (HCoV-OC43) spike protein (S1+S2 ECD) (AVR40344.1) (Met1-Pro1304) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: HCoV-OC43
• Tag: His
• Protein length: Met1-Pro1304
• Form: Lyophilized from sterile 20 mM PB, 300 mM NaCl, 10 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human coronavirus (HCoV-OC43) spike protein (S1+S2 ECD, His Tag) consists of 1303 amino acids and predicts a molecular mass of 145.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.