Recombinant COVID-19 Spike S1+S2 (157-158 deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R, D950N) protein(Met1-Pro1213), His&Avi-tagged, Biotinylated

• Cat.No.: Spike-1214V
• Product Overview: Recombinant SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations 157-158 deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R, D950N) (Met1-Pro1213) was expressed in Insect Cells with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617.2) which emerged in the India.

Specification

• Source: Insect Cells
• Species: COVID-19
• Tag: C-His&Avi
• Protein length: Met1-Pro1213
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 Spike S1+S2 consists of 1222 amino acids and predicts a molecular mass of 136.01 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2