Recombinant H9N2 HA protein(Met1-Lys523), His-tagged

• Cat.No.: HA-582V
• Product Overview: Recombinant influenza A H9N2 Hemagglutinin (A/Guinea fowl/Hong Kong/WF10/99 (H9N2)) (AAO46082.1)(Met 1-Lys 523) (HA1+HA2, uncleaved) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: H9N2
• Tag: His
• Protein length: 1-523 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant influenza A H9N2 HA (A/Guinea fowl/Hong Kong/WF10/99 (H9N2)) comprises 516 amino acids and has a predicted molecular mass of 58.2 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.