Active Recombinant H1N1 (A/California/06/2009) HA (ΔTM) Protein, His-tagged

• Cat.No.: HA-1863H
• Product Overview: Recombinant H1N1 (A/California/06/2009) HA (ΔTM) Protein(ACP41935.1) (Met1-Gln529) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect cells
• Species: H1N1
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells.
  HA titer is 5-50 μg/mL for 1%GRBC
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/California/06/2009) consists 523 amino acids and predicts a molecular mass of 58.9 kDa.
• Protein length: Met1-Gln529
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.