Recombinant H3N2 (A/Aichi/2/1968) M1 Protein, His-tagged

• Cat.No.: M1-722V
• Product Overview: Recombinant influenza A virus (A/Aichi/2/1968(H3N2)) matrix protein 1 (AFM71858.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag.

Specification

• Source: E. coli
• Species: H3N2
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile 1mM EDTA, 50mM Tris, 50mM NaCl, 5% glycerol, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant M1 subunit of influenza A H3N2 consists of 268 amino acids and has a predicted molecular mass of 30 kDa. It migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-252 a.a.