Recombinant H2N2 (A/Japan/305/1957) HA Protein, His-tagged

• Cat.No.: HA-299V
• Product Overview: Recombinant extracellular domain of Influenza A virus (A/Japan/305/1957 (H2N2)) (AAA43185.1) hemagglutinin (Met 1-Gln 525) (HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H2N2
• Source: HEK293
• Tag: His
• Protein Length: 1-525 a.a.
• Predicted N Terminal: Asp 16
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/Japan/305/1957 (H2N2)) comprises 521 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  Variation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins (2024)