Recombinant Full Length Yarrowia Lipolytica Chitobiosyldiphosphodolichol Beta-Mannosyltransferase(Alg1) Protein, His-Tagged

• Cat.No.: RFL28465YF
• Product Overview: Recombinant Full Length Yarrowia lipolytica Chitobiosyldiphosphodolichol beta-mannosyltransferase(ALG1) Protein (Q6C3K2) (1-463aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-463)
• AA Sequence: MKAWHWSVTLVVIYLAIPVILYLLTRKDDRKPLSDIRKRKRTIVLVLGDLGRSPRMLYHA RSLARSGHKVDLCGYDGAKPFDEILNNDLIKIHHIPLILNTRKLPFVVFGILKVIRQHWL LISLLYKLRGADYLLVQNPPSIPTLGVVRFYNLFLSTRTKVVLDWHNFGYTILALKLPET HPMVKFAKFYEGFFGGRAFVHLCVTVLMGQAMRKTFGMSGRRIVPLHDRPAFHFKPLSES EKLDVLRDFKETLYDDMTADHKIIVSSTSYTPDENFNILLDALALYDESKLDLPPLRVII TGKGPMMPEFLAKVEKLQLKRVSIRTAWLEFADYPRILGAAHLGVSLHESSSGYDLPMKV VDMFGCGIPVVSVDYAALSELVKTNTNGVAVKGHVEMGNTFMSLFSNRGKLDNIKRGAMI ESRNTWDQTWVKTVGPLFDIGEYVQQRPDEDYDFSSSSSDDDH
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: ALG1
• Synonyms: ALG1; YALI0E34133g; Chitobiosyldiphosphodolichol beta-mannosyltransferase; Asparagine-linked glycosylation protein 1; Beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransfera
• UniProt ID: Q6C3K2