Recombinant Full Length Mouse HTR2C Protein, His-tagged

• Cat.No.: HTR2C-001M
• Product Overview: Recombinant Full Length Mouse HTR2C Protein with N-10×His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-459aa
• Description: Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations.
• Tag: N-10×His
• Molecular Mass: 54.6 kDa
• AA Sequence: IVTDTFNSSDGGRLFQFPDGVQNWPALSIVVIIIMTIGGNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYFLTIYVLRRQTLMLLRGHTEEELRNISLNFLKCCCKKGDEEENAPNPNPDQKPRRKKKEKRPRGTMQAINNEKKASKVLGIVFFVFLIMWCPFFITNILSVLCGKACNQKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSKYLRCDYKPDKKPPVRQIPRVAATALSGRELNVNIYRHTNERVVRKANDTEPGIEMQVENLELPVNPSNVVSERISSV
• Purity: ≥ 85% by SDS-PAGE
• Storage: Store it at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4
  The volume before lyophilization is 103 μL/vial.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20/-80 centigrade. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: Htr2c 5-hydroxytryptamine (serotonin) receptor 2C [ Mus musculus (house mouse) ]
• Official Symbol: HTR2C
• Synonyms: HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C; 5-hydroxytryptamine receptor 2C; 5-HT1C; 5-HT-1C; 5-HT-2C; 5-HT2C receptor; serotonin receptor 2C; 5-hydroxytryptamine receptor 1C; SR1; 5HT1c; Htr1c; 5-HT2C; 5-HT2cR; 5-HTR2C
• Gene ID: 15560
• mRNA Refseq: NM_008312
• Protein Refseq: NP_032338
• UniProt ID: P34968