Recombinant Full Length Mesocricetus Auratus 3 Beta-Hydroxysteroid Dehydrogenase Type 3(Hsd3B3) Protein, His-Tagged

• Cat.No.: RFL16733MF
• Product Overview: Recombinant Full Length Mesocricetus auratus 3 beta-hydroxysteroid dehydrogenase type 3(HSD3B3) Protein (O35296) (2-373aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Species: Mesocricetus auratus
• Source: E.coli
• Tag: His
• Protein Length: Full Length of Mature Protein (2-373)
• Form: Lyophilized powder
• AA Sequence: PAWSCLVTGAGGFLGQRIIRMLAQEKELQEVRTLFRSFTPKHREELSKLQTKTKVTVLEG DILDAQCLRRACQGISVVIHTAAAIDVFGAIPRQTVIDINLKGTQHLLDACIGARVPVFI YSSSVAVAGPNSYKVIIQNGSEEENHESTWSDPYAYSKKMAEKAVLAANGSTLKDGGTLH TCALRLPFIYGEKSKFISDTMDRALKNNGLINGFSRFSVISSVYVNNAAWAHVLAARGLQ DPKKSPNIQGQFYYISDDTPHQSYDDLCYTLSKDWGLRPDSSWKPPVALLYWFGFLLETV SFLLRPVYNYQPPFNRHLVTLLNSVFTFSYKKAQRDLGYEPLVSWEEAREKTSEWIGSLV EQHKGTLNIKAQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: HSD3B3
• Synonyms: HSD3B3; NADPH-dependent 3-keto-steroid reductase HSD3B3; 3 beta-hydroxysteroid dehydrogenase type 3; 3 beta-hydroxysteroid dehydrogenase type III; 3 beta-HSD III; Dihydrotestosterone 3-ketoreductase
• UniProt ID: O35296