Recombinant Full Length Kluyveromyces Lactis Dol-P-Man:Man(5)Glcnac(2)-Pp-Dol Alpha-1,3-Mannosyltransferase(Alg3) Protein, His-Tagged

• Cat.No.: RFL7856KF
• Product Overview: Recombinant Full Length Kluyveromyces lactis Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase(ALG3) Protein (Q6CMF1) (1-462aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-462)
• AA Sequence: MVEEGANKTVGAPTVGDDQQPKEFVRPPFTPFQDILDAFNYLMWNPEANAIAMPVLILLE SIAMKFIQNKVSYTEIDYTAYMEQIWMIQNGERDYSQIKGGTGPLVYPAGHVFIYKIFEW VSDGLENISEAQDLFRYLYVITLMIQFMCFGLLNIPPGYAIFAILSKRLHSVYVLRLFND CFTTLFMSLAVLVMILCAKYKIRGFLVLIGSSFYSMAVSIKMNALLYLPGVLLTIYLLER CNTFKIVLNLAVMVIWQVIIAIPFWKEYPWEYLQSAFNFSRQFMYKWSVNWQMVDEEVFL DPLFHRSLLISHVIVLVVFLFYKLIPTNMNTPAGLLKIGKANLLHPFTDAVFSAMRVNAE QIAYILLVTNYIGVLFARSLHYQFLSWYHWTLPVLLNWANVPYPLCVLWYLTHEWCWNSY PPNATASTLLHACNTSLLLAVFLRGPANSKSGDNETTHEKAE
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: ALG3
• Synonyms: ALG3; KLLA0E20823g; Dol-P-Man:Man(5GlcNAc(2-PP-Dol alpha-1,3-mannosyltransferase; Asparagine-linked glycosylation protein 6; Dol-P-Man-dependent alpha(1-3-mannosyltransferase; Dolichyl-P-Man:Man(5GlcNAc(2-PP-dolichyl mannosyltransferase
• UniProt ID: Q6CMF1