Recombinant Full Length Human SMAD Family Member 4, His-tagged

• Cat.No.: SMAD4-386H
• Product Overview: Recombinant SMAD4 protein was expressed in E.coli and purified by using conventional chromatography techniques, 62.6 kDa (572 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-552 a.a.
• Description: SMAD4 belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD4 promotes the binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides the function of activation required for SMAD1 or SMAD2 to stimulate transcription and may also act as a tumor suppressor.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SMAD4 SMAD family member 4 [ Homo sapiens ]
• Synonyms: SMAD4; SMAD family member 4; JIP; DPC4; MADH4; mothers against decapentaplegic homolog 4;OTTHUMP00000163548; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; MAD, mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; hSMAD4; Deletion target in pancreatic carcinoma 4; MAD, mothers against decapentaplegic homolog 4 (Drosophila); Mothers against DPP homolog 4; SMAD, mothers against DPP homolog 4 (Drosophila)
• Gene ID: 4089
• mRNA Refseq: NM_005359
• Protein Refseq: NP_005350
• MIM: 600993
• UniProt ID: Q13485
• Chromosome Location: 18q21.1
• Pathway: Adherens junction; Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Pancreatic cancer; TGF-beta signaling pathway; Wnt signaling pathway; Signaling by BMP; Signaling by TGF beta
• Function: R-SMAD binding; collagen binding; promoter binding; protein homodimerization activity; transcription activator activity; transcription factor activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity