Recombinant Full Length Human RHOA Protein, His-tagged

• Cat.No.: RHOA-3975H
• Product Overview: Recombinant Human RHOA (P61586-1) (Met 1-Leu 193) was expressed in Insect Cells, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-193 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human RHOA consists of 212 amino acids and has a calculated molecular mass of 24 kDa. It migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RHOA ras homolog family member A [ Homo sapiens ]
• Official Symbol: RhoA
• Synonyms: RHOA; ras homolog family member A; ARH12, ARHA, ras homolog gene family, member A; transforming protein RhoA; Rho12; RhoA; RHOH12; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A; ARHA; ARH12; RHO12
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• UniProt ID: P61586