Active Recombinant Human RHOA protein, His-tagged

• Cat.No.: RHOA-33H
• Product Overview: Recombinant Human RHOA fused with histidine tag in the amino-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
• Form: The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
• Bio-activity: The biological activity of His-RhoA is determined from its ability to catalyze the exchange of GDP for GTP.
• Molecular Mass: The recombinant protein is approximately 27 kDa.
• Purity: 80%
• Applications: RhoA biochemistry;
  RhoA GTPase assays;
  RhoA nucleotide exchange assays;
  RhoA binding studies.

Gene Information

• Gene Name: RHOA ras homolog family member A [ Homo sapiens ]
• Official Symbol: RHOA
• Synonyms: RHOA; ras homolog family member A; ARH12, ARHA, ras homolog gene family, member A; transforming protein RhoA; Rho12; RhoA; RHOH12; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A; ARHA; ARH12; RHO12
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• UniProt ID: P61586
• Chromosome Location: 3p21.3
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding