Recombinant Full Length Human PRKG1, GST-tagged, Active

• Cat.No.: PRKG1-413H
• Product Overview: Recombinant full-length human PRKG1 was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=100kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PRKG1 protein kinase, cGMP-dependent, type I [ Homo sapiens ]
• Synonyms: PRKG1; protein kinase, cGMP-dependent, type I; PGK; PKG; CGKI; PRKG1B; PRKGR1B; FLJ36117; MGC71944; cGKI-BETA; cGKI-alpha; DKFZp686K042; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; EC 2.7.11.12
• Gene ID: 5592
• mRNA Refseq: NM_001098512
• Protein Refseq: NP_001091982
• MIM: 176894
• UniProt ID: Q13976
• Chromosome Location: 10q11.2
• Pathway: Gap junction; Long-term depression; Olfactory transduction; Vascular smooth muscle contraction
• Function: ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity