Recombinant Full Length Human PRKAR1A, His tagged

• Cat.No.: PRKAR1A-333H
• Product Overview: Recombinant Human PRKAR1A (P10644) (Met1-Val381), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• ProteinLength: 1-381 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PRKAR1A consists of 391 amino acids and predicts a molecular mass of 44.3 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) [ Homo sapiens ]
• Official Symbol: PRKAR1A
• Gene ID: 5573
• mRNA Refseq: NM_002734
• Protein Refseq: NP_002725
• MIM: 188830
• UniProt ID: P10644
• Chromosome Location: 17q23-q24