Recombinant Full Length Human NAE1, His-tagged

• Cat.No.: NAE1-26749TH
• Product Overview: Recombinant full length Human APPBP1 with N terminal His tag; 557 amino acids with tag, Predicted MWt 62.7 kDa.

Specification

• Description: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimers disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
• Protein length: 1-534aa
• Conjugation: HIS
• Molecular Weight: 62.700kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Ubiquitous in fetal tissues. Expressed throughout the adult brain.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.03% DTT, 10% Glycerol, 1.17% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSMAQLGKLLKEQKYDRQL RLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSF TIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELN SDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSL RLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPD NALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVI IAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENG APEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINI TKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSG KYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISE KELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMD NPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKL KSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFL GGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL
• Sequence Similarities: Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Gene Information

• Gene Name: NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens ]
• Official Symbol: NAE1
• Synonyms: NAE1; NEDD8 activating enzyme E1 subunit 1; amyloid beta precursor protein binding protein 1 , amyloid beta precursor protein binding protein 1, 59kDa , amyloid beta precursor protein binding protein 1, 59kD , APPBP1; NEDD8-activating enzyme E1 regulato
• Gene ID: 8883
• mRNA Refseq: NM_001018159
• Protein Refseq: NP_001018169
• MIM: 603385
• Uniprot ID: Q13564
• Chromosome Location: 16q22
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem
• Function: contributes_to NEDD8 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein heterodimerization activity