Recombinant Full Length Human MRPL35 Protein, GST-tagged

• Cat.No.: MRPL35-6453HF
• Product Overview: Human MRPL35 full-length ORF ( NP_057706.2, 1 a.a. - 188 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 47.9 kDa
• Protein length: 188 amino acids
• AA Sequence: MAASAFAGAVRAASGILRPLNILASSTYRNCVKNASLISALSTGRFSHIQTPVVSSTPRLTTSERNLTCGHTSVILNRMAPVLPSVLKLPVRSLTYFSARKGKRKTVKAVIDRFLRLHCGLWVRRKAGYKKKLWKKTPARKKRLREFVFCNKTQSKLLDKMTTSFWKRRNWYVDDPYQKYHDRTNLKV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MRPL35 mitochondrial ribosomal protein L35 [ Homo sapiens ]
• Official Symbol: MRPL35
• Synonyms: MRPL35; mitochondrial ribosomal protein L35; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35
• Gene ID: 51318
• mRNA Refseq: NM_016622
• Protein Refseq: NP_057706
• MIM: 611841
• UniProt ID: Q9NZE8