Recombinant Full Length Human ILK associated serine/threonine phosphatase Protein, His tagged

• Cat.No.: ILKAP-147H
• Product Overview: Recombinant human ILKAP protein (Q9H0C8) (Met 1-His 392) with a His tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn2+ ions but inhibited by Mg2+ and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.
• Source: E. coli
• Species: Human
• Tag: N-His and C-His
• Protein length: 1-392aa
• Molecular Mass: 44.6 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: ILKAP ILK associated serine/threonine phosphatase [ Homo sapiens (human) ]
• Official Symbol: ILKAP
• Synonyms: ILKAP; integrin-linked kinase-associated serine/threonine phosphatase; integrin linked kinase associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase 2C; DKFZP434J2031; FLJ10181; protein phosphatase 2c, delta isozyme; integrin-linked kinase associated phosphatase; ILKAP2; ILKAP3; PP2C-DELTA; MGC4846; DKFZp434J2031
• Gene ID: 80895
• mRNA Refseq: NM_030768
• Protein Refseq: NP_110395
• MIM: 618909
• UniProt ID: Q9H0C8