Recombinant Full Length Human IFIH1 Protein, His tagged

• Cat.No.: IFIH1-01HFL
• Product Overview: Full-length cDNA coding for the human melanoma differentiation-associated protein 5 fused to a deca-histidine purification tag was expressed in baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.

Specification

• Species: Human
• Source: Baculovirus
• Tag: His
• ProteinLength: 1-1025 aa
• Description: IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome.
• Tag: His
• Calculated isoelectric point: pH 5.60
• Molecular Mass: 118 kDa
• Immunological function: Binds IgG-type human auto-antibodies.
• Immunological tests: Immunodot analyses with positive/negative samples.
• Biochemical tests: SDS-PAGE (purity > 80%); Western blot with i: anti-MDA5 auto-antibody positive sample; ii: monoclonal anti-His-tag antibody.
• Storage: At -70 centigrade or below. Repeated freeze/thaw cycles should be avoided.
• Storage Buffer: Neutral to slightly alkaline pH and 6M urea.

Gene Information

• Gene Name: IFIH1 interferon induced with helicase C domain 1 [ Homo sapiens (human) ]
• Official Symbol: IFIH1
• Synonyms: IFIH1; interferon induced with helicase C domain 1; interferon-induced helicase C domain-containing protein 1; helicard; Hlcd; IDDM19; MDA 5; MDA5; CADM-140 autoantigen; helicase with 2 CARD domains; RNA helicase-DEAD box protein 116; murabutide down-regulated protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; melanoma differentiation associated protein-5; melanoma differentiation-associated protein 5; interferon-induced with helicase C domain protein 1; clinically amyopathic dermatomyositis autoantigen 140 kDa; MDA-5; MGC133047
• Gene ID: 64135
• mRNA Refseq: NM_022168
• Protein Refseq: NP_071451
• MIM: 606951
• UniProt ID: Q9BYX4