Recombinant Full Length Human grancalcin, EF-hand calcium binding protein Protein, His&Flag&StrepII tagged

• Cat.No.: GCA-036H
• Product Overview: Recombinant human Grancalcin/GCA protein (P28676) (Met 1-Ile 217) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 1-217aa
• Molecular Mass: 30.7 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: GCA grancalcin, EF-hand calcium binding protein [ Homo sapiens (human) ]
• Official Symbol: GCA
• Synonyms: GCA; grancalcin, EF-hand calcium binding protein; grancalcin, EF hand calcium binding protein; grancalcin; GCL; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein
• Gene ID: 25801
• mRNA Refseq: NM_012198
• Protein Refseq: NP_036330
• MIM: 607030
• UniProt ID: P28676