Recombinant Full Length Human glial fibrillary acidic Protein, Tag Free

• Cat.No.: GFAP-31H
• Product Overview: This product is identical to the human GFAP isotype I sequence in GenBank entry NP_002046.1. It is widely used as a standard in ELISA and other antibody based assays. The human GFAP protein is a little different in amino acid sequence from that of the rat protein, so a recombinant form of the rat protein is also available from EnCor, Prot-r-GFAP-rat.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-432aa
• Description: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Tag: Non
• Molecular Mass: 50 kDa by SDS-PAGE plus about 5 kDa tag sequence
• Applications: Protein standard for ELISA, MSD, Luminex and Simoa assays, immunogen for antibody production
• Storage: Stable at 4 centigrade for several months. For longer term store at -20 centigrade or lower.
• Storage Buffer: 6M Urea, 10mM phosphate pH=7.5
• Concentration: 1 mg/mL
• References: 1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
  2. Brenner M, et al. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001).
  3. Silver J. Miller JH. Regeneration beyond the glial scar. Nat. Rev. Neurosci. 5:146-56 (2004).
  4. Schiff L1, Hadker N, Weiser S, Rausch C. A literature review of the feasibility of glial fibrillary acidic protein as a biomarker for stroke and traumatic brain injury. Mol. Diagn. Ther. 16:79-92 (2012).

Gene Information

• Gene Name: GFAP glial fibrillary acidic protein [ Homo sapiens (human) ]
• Official Symbol: GFAP
• Synonyms: GFAP; glial fibrillary acidic protein; FLJ45472; intermediate filament protein; FLJ42474
• Gene ID: 2670
• mRNA Refseq: NM_002055
• Protein Refseq: NP_002046
• MIM: 137780
• UniProt ID: P14136