Recombinant Full Length Human FGF23 Protein, C-His-tagged

• Cat.No.: FGF23-299H
• Product Overview: Recombinant Full Length Human FGF23 Protein, fused to His-tag at C-terminus, was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-251 aa
• Description: FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).
• Form: Lyophilized. Contains PBS.
• Bio-activity: Activates ERK and FRS2α phosphorylation in Klotho expressing cells.
• Molecular Mass: ~30kDa (SDS-PAGE, full-length). FGF23 is cleaved between Arg179 and Ser180 (two bands of ~14kDa and ~16kDa).
• Endotoxin: <0.01EU/μg purified protein (LAL test; Lonza).
• Purity: ≥90% (SDS-PAGE)
• Storage: Short Term Storage: 4 centigrade
  Long Term Storage: -20 centigrade
• Shipping: BLUE ICE
• Handling Advice: After reconstitution, prepare aliquots and store at -20 centigrade.
  Avoid freeze/thaw cycles.
  Centrifuge lyophilized vial before opening and reconstitution.
  PBS containing at least 0.1% BSA should be used for further dilutions.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20 centigrade.
  Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Reconstitution: Reconstitute with 100μl sterile water.
• References: 1. Maternally-derived antibody to fibroblast growth factor-23 reduced dietary phosphate requirements in growing chicks: E.A. Bobeck, et al.; BBRC 420, 666 (2012)
  2. Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens: Z. Ren, et al.; Poultry Science 96, 127 (2017).

Gene Information

• Gene Name: FGF23 fibroblast growth factor 23 [ Homo sapiens (human) ]
• Official Symbol: FGF23
• Synonyms: FGF23; fibroblast growth factor 23; Fibroblast Growth Factor 23; Tumor-derived Hypophosphatemia-inducing Factor; ADHR; FGFN; HYPF; HFTC2; HPDR2; PHPTC
• Gene ID: 8074
• mRNA Refseq: NM_020638.3
• Protein Refseq: NP_065689.1
• MIM: 605380
• UniProt ID: Q9GZV9