Recombinant Full Length Human eukaryotic translation initiation factor 3, subunit K Protein, His&Flag&StrepII tagged

• Cat.No.: EIF3K-018H
• Product Overview: Recombinant human EIF3K protein (Q9UBQ5) (Met 1-Gln 218) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 1-218aa
• Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 31.7 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: EIF3K eukaryotic translation initiation factor 3, subunit K [ Homo sapiens (human) ]
• Official Symbol: EIF3K
• Synonyms: EIF3K; eukaryotic translation initiation factor 3, subunit K; EIF3S12, eukaryotic translation initiation factor 3, subunit 12; eukaryotic translation initiation factor 3 subunit K; ARG134; eIF3k; HSPC029; M9; PLAC 24; PRO1474; PTD001; eIF-3 p25; eIF-3 p28; muscle specific; muscle-specific gene M9 protein; eukaryotic translation initiation factor 3 subunit 12; eukaryotic translation initiation factor 3, subunit 12; PLAC24; EIF3S12; MSTP001; PLAC-24; EIF3-p28
• Gene ID: 27335
• mRNA Refseq: NM_013234
• Protein Refseq: NP_037366
• MIM: 609596
• UniProt ID: Q9UBQ5