Recombinant Full Length Human Dopamine beta-Hydroxylase Protein, His tagged

• Cat.No.: DBH-23HFL
• Product Overview: Recombinant Full Length Human Dopamine beta-Hydroxylase Protein (Met1-Gly617) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: The product is shipped with polar packs.
• Source: CHO
• Species: Human
• Tag: C-His
• Protein length: 1-617aa
• Bio-activity: Measured by its ability to convert tyramine to octopamine. The specific activity is > 7, 000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 66 kDa
• N-terminal Sequence Analysis: Leu37 & Ser40
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• References: 1. Dhawan, S. et al. (1987) J. Biol. Chem. 262:1869.
  2. Saxena, A. and P.J. Fleming (1983) J. Biol. Chem. 258:4147.
  3. Levin, E.Y. et al. (1960) J. Biol. Chem. 235:2080.

Gene Information

• Gene Name: DBH dopamine beta-hydroxylase (dopamine beta-monooxygenase) [ Homo sapiens (human) ]
• Official Symbol: DBH
• Synonyms: DBH; dopamine beta-hydroxylase (dopamine beta-monooxygenase); dopamine beta-hydroxylase; DBM
• Gene ID: 1621
• mRNA Refseq: NM_000787
• Protein Refseq: NP_000778
• MIM: 609312
• UniProt ID: P09172