Recombinant Full Length Human DDX3Y Protein, His-tagged

• Cat.No.: DDX3Y-3751H
• Product Overview: Recombinant Full Length Human DDX3Y Protein (1-660 aa) with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-660 aa
• Description: The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Tag: His
• Molecular Mass: ~ 74 kDa
• AA Sequence: HHHHHHMSHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIPIIKGKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAVKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEDLDKRSFLLDILGATGSDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNMNITKDLLDLLVEAKQEVPSWLENMAYEHHYKGGSRGRSKSNRFSGGFGARDYRQSSGSSSSGFGASRGSSSRSGGGGYGNSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN
• Endotoxin: < 10 EU/mL (determined by the LAL method)
• Purity: ≥ 90% by SDS-PAGE
• Storage: Long Term Storage at -20 centigrade to -80 centigrade. Avoid freeze/thaw cycles.
• Storage Buffer: PBS buffer
• Concentration: 0.15 mg/mL

Gene Information

• Gene Name: DDX3Y DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked [ Homo sapiens (human) ]
• Official Symbol: DDX3Y
• Synonyms: DDX3Y; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked; DBY, DEAD/H (Asp Glu Ala Asp/His) box polypeptide, Y chromosome; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3, Y-chromosomal; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome; DBY
• Gene ID: 8653
• mRNA Refseq: NM_001122665
• Protein Refseq: NP_001116137
• MIM: 400010
• UniProt ID: O15523