Recombinant Full Length Human DDX25 Protein, GST-tagged

• Cat.No.: DDX25-2564HF
• Product Overview: Human DDX25 full-length ORF ( AAH50360, 1 a.a. - 483 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 78.87 kDa
• Protein length: 483 amino acids
• AA Sequence: MASLLWGGDAGAAESERLNSHFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DDX25 DEAD (Asp-Glu-Ala-Asp) box helicase 25 [ Homo sapiens ]
• Official Symbol: DDX25
• Synonyms: DDX25; DEAD (Asp-Glu-Ala-Asp) box helicase 25; DEAD (Asp Glu Ala Asp) box polypeptide 25 , DEAD/H (Asp Glu Ala Asp/His) box polypeptide 25; ATP-dependent RNA helicase DDX25; gonadotropin regulated testicular RNA helicase; GRTH; DEAD box protein 25; DEAD (Asp-Glu-Ala-Asp) box polypeptide 25; gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25
• Gene ID: 29118
• mRNA Refseq: NM_013264
• Protein Refseq: NP_037396
• MIM: 607663
• UniProt ID: Q9UHL0