Recombinant Full Length Human DCTN2 Protein, GST-tagged

• Cat.No.: DCTN2-2939HF
• Product Overview: Human DCTN2 full-length ORF ( AAH00718.1, 1 a.a. - 401 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 70.6 kDa
• Protein length: 401 amino acids
• AA Sequence: MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DCTN2 dynactin 2 (p50) [ Homo sapiens ]
• Official Symbol: DCTN2
• Synonyms: DCTN2; dynactin 2 (p50); dynactin subunit 2; DCTN 50; RBP50; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide; DCTN50; DYNAMITIN
• Gene ID: 10540
• mRNA Refseq: NM_006400
• Protein Refseq: NP_006391
• MIM: 607376
• UniProt ID: Q13561