Recombinant Full Length Human CHI3L1 Protein, His tagged

• Cat.No.: CHI3L1-03HFL
• Product Overview: Recombinant human CHI3L1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-383 aa
• Description: Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
• Form: Liquid
• Molecular Mass: 41.4 kDa (370aa)
• AA Sequence: YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRGDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
• Biotin binding Site: 0
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1 mM DTT
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Reference: 1. Hakala BE., et al. (1993) J Biol Chem 268:25803-25810. 2. Eurich K., et al. (2009) World J Gastroenterol. 15:5249-5259.

Gene Information

• Gene Name: CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39) [ Homo sapiens (human) ]
• Official Symbol: CHI3L1
• Synonyms: CHI3L1; chitinase 3-like 1 (cartilage glycoprotein-39); chitinase-3-like protein 1; GP39; YKL40; 39 kDa synovial protein; cartilage glycoprotein 39; ASRT7; GP-39; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; FLJ38139; DKFZp686N19119
• Gene ID: 1116
• mRNA Refseq: NM_001276
• Protein Refseq: NP_001267
• MIM: 601525
• UniProt ID: P36222