Recombinant Full Length Human BARD1 Protein, Fc tagged

• Cat.No.: BARD1-03HF
• Product Overview: Recombinant Full Length Human BARD1 Protein with Fc tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: Fc
• Molecular Mass: The protein has a calculated MW of 113 kDa.
• AA Sequence: MPDNRQPRNRQPRIRSGNEPRSAPA MEPDGRGAWAHSRAALDRLEKLLRC SRCTNILREPVCLGGCEHIFCSNCV SDCIGTGCPVCYTPAWIQDLKINRQ LDSMIQLCSKLRNLLHDNELSDLKE DKPRKSLFNDAGNKKNSIKMWFSPR SKKVRYVVSKASVQTQPAIKKDASA QQDSYEFVSPSPPADVSERAKKASA RSGKKQKKKTLAEINQKWNLEAEKE DGEFDSKEESKQKLVSFCSQPSVIS SPQINGEIDLLASGSLTESECFGSL TEVSLPLAEQIESPDTKSRNEVVTP EKVCKNYLTSKKSLPLENNGKRGHH NRLSSPISKRCRTSILSTSGDFVKQ TVPSENIPLPECSSPPSCKRKVGGT SGRKNSNMSDEFISLSPGTPPSTLS SSSYRRVMSSPSAMKLLPNMAVKRN HRGETLLHIASIKGDIPSVEYLLQN GSDPNVKDHAGWTPLHEACNHGHLK VVELLLQHKALVNTTGYQNDSPLHD AAKNGHVDIVKLLLSYGASRNAVNI FGLRPVDYTDDESMKSLLLLPEKNE SSSASHCSVMNTGQRRDGPLVLIGS GLSSEQQKMLSELAVILKAKKYTEF DSTVTHVVVPGDAVQSTLKCMLGIL NGCWILKFEWVKACLRRKVCEQEEK YEIPEGPRRSRLNREQLLPKLFDGC YFYLWGTFKHHPKDNLIKLVTAGGG QILSRKPKPDSDVTQTINTVAYHAR PDSDQRFCTQYIIYEDLCNYHPERV RQGKVWKAPSSWFIDCVMSFELLPL DSGGGGSGGGGSGGGGSEPKSCDKT HTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTPEVTCVVVDVSHEDPEV KFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTISKAKGQPREPQV YTLPPSRDELTKNQVSLTCLVKGFY PSDIAVEWESNGQPENNYKTTPPVL DSDGSFFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK
• Purity: > 80% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.44 mg/mL by BCA
• Storage Buffer: Sterile PBS, pH 7.4, 1% SKL

Gene Information

• Gene Name: BARD1 BRCA1 associated RING domain 1 [ Homo sapiens (human) ]
• Official Symbol: BARD1
• Synonyms: BARD1; BRCA1 associated RING domain 1; BRCA1-associated RING domain protein 1; BARD-1; BRCA1-associated RING domain gene 1; BRCA1 associated RING domain 1 isoform eta; BRCA1 associated RING domain 1 isoform phi; BRCA1 associated RING domain 1 isoform alfa; BRCA1 associated RING domain 1 isoform beta; BRCA1 associated RING domain 1 isoform delta; BRCA1 associated RING domain 1 isoform gamma; BRCA1 associated RING domain 1 isoform epsilon
• Gene ID: 580
• mRNA Refseq: NM_000465
• Protein Refseq: NP_000456
• MIM: 601593
• UniProt ID: Q99728

Reviews

02/06/2022

Researchers highly recommend the BARD1 protein due to its excellent performance in ELISA and its suitability for protein electron microscopy structure analysis.

04/27/2019

The BARD1 protein comes highly recommended for a variety of applications, particularly in ELISA and protein electron microscopy structure analysis.

12/17/2018

Researchers can rely on the BARD1 protein's specificity and sensitivity to obtain accurate and reliable results.

Q&As

How does BARD1 function in DNA repair?

BARD1 works in conjunction with BRCA1 to repair damaged DNA by facilitating homologous recombination, a crucial mechanism for DNA repair.

How does BARD1 impact tumor progression?

Dysregulated BARD1 expression or function can contribute to uncontrolled cell growth and tumor progression by compromising DNA repair mechanisms.

Are there diagnostic tests for BARD1 mutations?

Genetic testing can identify mutations in the BARD1 gene, aiding in assessing an individual's predisposition to certain cancers.

How does BARD1 relate to BRCA1 in cancer risk?

BARD1 forms a complex with BRCA1, and mutations in either gene can compromise DNA repair, elevating the risk of cancer development.

Apart from cancer, what other conditions might BARD1 be involved in?

BARD1 is also being studied in relation to its role in other diseases, including neurodegenerative disorders and infertility.