Recombinant Full Length Human ALAD Protein, GST-tagged

• Cat.No.: ALAD-1387HF
• Product Overview: Human ALAD full-length ORF ( NP_000022.2, 1 a.a. - 339 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 63.6 kDa
• Protein length: 339 amino acids
• AA Sequence: MPLCPLAHAM QPQSVLHSGY FHPLLRAWQT ATTTLNASNL IYPIFVTDVP DDIQPITSLP GVARYGVKRL EEMLRPLVEE GLRCVLIFGV PSRVPKDERG SAADSEESPA IEAIHLLRKT FPNLLVACDV CLCPYTSHGH CGLLSENGAF RAEESRQRLA EVALAYAKAG CQVVAPSDMM DGRVEAIKEA LMAHGLGNRV SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR GLALRAVDRD VREGADMLMV KPGMPYLDIV REVKDKHPDL PLAVYHVSGE FAMLWHGAQA GAFDLKAAVL EAMTAFRRAG ADIIITYYTP QLLQWLKEE
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ALAD aminolevulinate dehydratase [ Homo sapiens ]
• Official Symbol: ALAD
• Synonyms: ALAD; aminolevulinate dehydratase; aminolevulinate, delta , dehydratase; delta-aminolevulinic acid dehydratase; ALADH; PBGS; porphobilinogen synthase; aminolevulinate, delta-, dehydratase; MGC5057
• Gene ID: 210
• mRNA Refseq: NM_000031
• Protein Refseq: NP_000022
• MIM: 125270
• UniProt ID: P13716