Recombinant Full Length Dictyostelium Discoideum Gdp-Man:Man(3)Glcnac(2)-Pp-Dol Alpha-1,2-Mannosyltransferase(Alg11) Protein, His-Tagged

• Cat.No.: RFL2238DF
• Product Overview: Recombinant Full Length Dictyostelium discoideum GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase(alg11) Protein (Q54DM9) (1-505aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Dictyostelium discoideum (Slime mold)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-505)
• AA Sequence: MDELIMMVFVLFTIVLLLTVSMTLAALISTIVVLVVPLIICIIALLIALRIKYGSKKDLS EVSIGFFHPYCTAGGGGERVLWCAIKSIQEEYPYVRCVVYTGDKESDDEIFNKVKKTFDI ELGRDNLEFIRLKKRKWVEASTYPRFTLIGQSLGSMILGWEALTKFVPTIFLDSMGYAFT FPIFSLIGGSTVACYVHYPTISSDMISSVKSSSYSFNNDVSISSNKFKTISKLIYYNIFS KIYQIVGSFSKLVMVNGTWTGNHIRDIWKKQFGYDLFIVYPPVDVKGRKQLKLGWMDGTR KNMILSIAQFRPEKNHQLQLRTLAHLLEKYPSHREQPLNTKLVLVGGVRDQADRDRVEQL RNLSKELNIEDHVEFQIGISSDQLNQLLSEASVGIHTMYNEHFGIGVVELMAAGVIPVAN NSAGPKEDIVRHEDTGFLASTIQEYAEYIHEILAYREKYVEMQKKARDSTDRFSESNFSN QFLKYIKPLINQSLRNNNSSSKKRN
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: alg11
• Synonyms: alg11; DDB_G0292118; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Asparagine-linked glycosylation protein 11 homolog; Glycolipid 2-alpha-mannosyltransferase
• UniProt ID: Q54DM9