Recombinant Full Length Cocksfoot Mottle Virus Replicase Polyprotein P2Ab(Orf2A-2B) Protein, His-Tagged

• Cat.No.: RFL15206CF
• Product Overview: Recombinant Full Length Cocksfoot mottle virus Replicase polyprotein P2AB(ORF2A-2B) Protein (Q0PW25) (398-942aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Species: Cocksfoot mottle virus (isolate Dactylis glomerata/Norway/CfMV-NO/1995) (CfMV)
• Source: E.coli
• Tag: His
• ProteinLength: Full Length of Mature Protein (398-942)
• Form: Lyophilized powder
• AA Sequence: TAIRPLNLPAGGLPTGQSALGQLIEYAGYVWRDEGIINSNGMPFRSAGKSSCRFREAVCR AVHRDVRAAETEFPELKELAWPSRGSKAEIGSLLFQAGRFERVEAPANLQLAITNLQAQY PRSRPRSCFRREPWCREDFVAEIEKIAHSGEINLKASPGVPLAEIGVSNQQVIDVAWPLV CEAVVERLHALASVDPRQHDWSPEELVKRGLCDPVRLFVKQEPHSRQKIEQGRFRLISSV SLVDQLVERMLFGPQNTTEIALWHSNPSKPGMGLSKASQVALLWEDLARKHQTHPGAMAD ISGFDWSVQDWELWADVSMRIELGSFPALMAKAAISRFYCLMNATFQLTNGELLTQELPG LMKSGSYCTSSSNSRIRCLMAELIGSPWCIAMGDDSVEGWVDDAPRKYSALGHLCKEYEA CPVLPNGDLKEVSFCSHLISKGRAELETWPKCLFRYLSGPHDVESLEMELSSSRRWGQIV RYLRRIGRVSGNDGEERSSNESPATTKTQGSAAAWGPPQEAWPVDGASLSTFEPSSSGWF HLEGW
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: ORF2A-2B
• Synonyms: ORF2A-2B; Replicase polyprotein P2AB
• UniProt ID: Q0PW25