Recombinant FIV p15 Antigen, His tagged

• Cat.No.: p15-081F
• Product Overview: p15 is the FIV N-terminal subunit of the retroviral gag polyprotein and has been expressed as a recombinant antigen fused to a trxA and a his-tag in its N terminus.

Specification

• Species: FIV
• Source: E.coli
• Tag: His
• Description: p15 is the feline immunodeficiency virus''s matrix protein and corresponds to the retroviral Gag polyprotein''s N-terminal subunit. This Gag protein is involved in the architecture of the viral particle, and it is essential for the infectivity of the virus. Corresponds to the p17 in HIV and SIV, which is ultimately localized to the inner surface of the virion membrane.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, is at the molecular markers of 35 kDa, while relative molecular mass calculated from amino acid sequence is 35577.43 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecylether
• Concentration: 1.23 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Serrière, J., Robert, X., Perez, M., Gouet, P. and C. Guillon. Biophysical characterization and crystal structure of 1.1. Feline Immunodeficiency Virus p15 matrix protein. 2013 Retrovirology, 10:64.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.