Recombinant Escherichia coli RPLF Protein (50S) (2-175 aa), GST-tagged

• Cat.No.: RPLF-1237E
• Product Overview: Recombinant Escherichia coli (strain K12) RPLF Protein (50S) (2-175 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Partial.

Specification

• Species: E.coli
• Source: E.coli
• Tag: GST
• Protein Length: 2-175 aa
• Description: This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.Gentamicin-resistant mutations in this protein affect translation fidelity.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 45.5 kDa
• AA Sequence: SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: rplF 50S ribosomal subunit protein L6 [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: RPLF
• Synonyms: ECK3292
• Gene ID: 947803
• Protein Refseq: NP_417764
• UniProt ID: P0AG55