Recombinant Enhanced Green Fluorescent Protein, His-tagged, Biotinylated

• Cat.No.: EGFP-01
• Product Overview: Biotin-conjugated recombinant human enhanced green fluorescent protein with N-terminal histidine tag was expressed in Human Embryonic Kidney 293 Cells.

Specification

• Species: Artificial sequence
• Source: HEK293
• Tag: His
• Description: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
• Form: Lyophilized powder
• AA Sequence: Full legnth-MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
• Purity: > 95%
• Applications: Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
• Shipping: Ice packs

Gene Information

• Official Symbol: EGFP
• Synonyms: EGFP; Enhanced Green Fluorescent Protein