Recombinant EBOV (subtype Sudan, strain Gulu) GP Protein, His-tagged

• Cat.No.: GP-613V
• Product Overview: Recombinant Sudan ebola virus (strain Uganda-00) GP (Q7T9D9) (Met1-Asp320) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: EBOV
• Source: HEK293
• Tag: His
• Predicted N Terminal: Met 33
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant Sudan ebola virus (strain Uganda-00) GP comprises 299 amino acids and has a predicted molecular mass of 33.8 kDa. The apparent molecular mass of the protein is approximately 46-64 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: GP virion spike glycoprotein precursor [ Zaire ebolavirus ]
• Official Symbol: GP
• Synonyms: spike glycoprotein; small secreted glycoprotein; second secreted glycoprotein
• Gene ID: 911829
• Protein Refseq: NP_066246
• UniProt ID: Q05320