Recombinant Ebolavirus (subtype Bundibugyo,strain Uganda 2007) Envelope Glycoprotein 1 (GP1) protein, His-tagged

• Cat.No.: GP1-353E
• Product Overview: Recombinant Ebolavirus (subtype Bundibugyo,strain Uganda 2007) Envelope Glycoprotein 1 (GP1) protein (Ile33-Gln304), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: EVD
• Source: HEK293
• Tag: His
• ProteinLength: 272
• Description: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly. GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: GP
• Official Symbol: GP
• Synonyms: BDBVp4
• Gene ID: 9487265
• Protein Refseq: YP_003815435.1
• UniProt ID: B8XCN0