Recombinant Ebola Virus (subtype Zaire,strain Kikwit-95) GP Protein, His-tagged

• Cat.No.: GP-634E
• Product Overview: Recombinant Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) Envelope Glycoprotein (GP), Ile 33-Asp 637 (Accession # AAQ55048.1) was produced in human 293 cells (HEK293).

Specification

• Species: EBOV
• Source: HEK293
• Tag: His
• Protein Length: 33-637 a.a.
• Description: Ebola virus envelope glycoprotein is initially produced as a precursor known as pre-GP, which is cleaved by furin into two subunits, GP1 and GP2, which remain associated through a disulfide linkage between Cys53 of GP1 and Cys609 of GP2. This heterodimer assembles into a 450-kDa trimer at the surface of nascent virions.
• Purity: >85% pure by SDS-PAGE
• Storage: Store lyophilized product at 4 centigrade for short term, or frozen at -20 centigrade to -80 centigrade for long term.
• Reconstitution: It is recommended to reconstitute the protein by adding 500ul sterile water to a stock solution of 200μg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. We recommend the addition of carrier protein (0.1% (w/v) BSA) for further dilution and long term storage.

Gene Information

• Gene Name: GP spike glycoprotein [ Zaire ebolavirus ]
• Official Symbol: GP
• Synonyms: GP; spike glycoprotein; small secreted glycoprotein; second secreted glycoprotein; envelope glycoprotein
• Gene ID: 911829
• mRNA Refseq: AY354458.1
• Protein Refseq: AAQ55048.1
• UniProt ID: Q05320