Recombinant E. coli RuvA Protein

• Cat.No.: ruvA-01E
• Product Overview: Recombinant E. coli tag-free RuvA Protein

Specification

• Species: E.coli
• Description: Binds Holliday junction as two tetramers (an octamer) and each tetrameric half recruits one RuvB hexameric helicase. RuvABC is an enzymatic complex which functions in recombination pathways to by binding to recombinational junctions and catalyzing strand cleavage and branch migration.
• Molecular Mass: 22 kD
• Purity: RuvA protein over 90% by SDS-PAGE (CBB staining)
• Applications: Functional as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein.
  For SNP analysis
• Storage: Ship at 4 centigrade or -20 centigrade. Spin-down and store at -20 or -80 centigrade for longer period.
• Concentration: 2.7 mg/mL (determined by BCA method)
• Storage Buffer: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol

Gene Information

• Gene Name: ruvA Holliday junction branch migration complex subunit RuvA [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: ruvA
• Synonyms: ruvA; Holliday junction branch migration complex subunit RuvA; ECK1862; Holliday junction branch migration complex subunit RuvA
• Gene ID: 946369
• Protein Refseq: NP_416375
• UniProt ID: P0A809