Recombinant E.coli RecA
Cat.No. : | recA-57E |
Product Overview : | A recombinant E. coli strain overexpressing E. coli recA from a plasmid. |
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Species : | E.coli |
Source : | E.coli |
Tag : | Non |
Description : | RecA functions in DNA recombination and DNA repair. RecA binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex can align with complementary double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non-hydrolyzable ATP analogs. The RecA-ATP-single stranded DNA complex also can function as a coprotease factor in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. RecA complexed with site-specific oligonucleotides have been used to target and specifically cleave large DNA fragments. |
Molecular Mass : | 37,973 Daltons |
Purity : | >95% by SDS-PAGE |
Unit Definition : | Sold by mass of pure protein determined at OD280 (A280 = 0.516 at 1 mg/mL, 1cm).. |
Storage : | -25 to -15°C |
Concentration : | 2.0 mg/mL |
Storage Buffer : | Supplied in: 10mM Tris-HCl, 1mM DTT. 0.1 mM EDTA 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X RecA Reaction Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C. |
Gene Name | recA?DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor [Escherichia coli str. K-12 substr. MG1655?] |
Official Symbol | recA |
Synonyms | recA; DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor; NP_417179.1; DNA strand exchange and renaturation, DNA-dependent ATPase, DNA- and ATP-dependent coprotease |
Gene ID | 947170 |
Protein Refseq | NP_417179 |
Pathway | Homologous recombination |
Function | peptidase activity |
◆ Recombinant Proteins | ||
RECA-477S | Recombinant Streptomyces coelicolor A3(2) RECA protein, His-tagged | +Inquiry |
recA-129E | Recombinant E. coli RecA | +Inquiry |
recA-57E | Recombinant E.coli RecA | +Inquiry |
RECA-2838S | Recombinant Staphylococcus epidermidis ATCC 12228 RECA protein, His-tagged | +Inquiry |
RECA-0087B | Recombinant Bacillus subtilis RECA protein, His-tagged | +Inquiry |
Not For Human Consumption!
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