Recombinant E.coli pykF, His-tagged

• Cat.No.: pykF-15843E
• Product Overview: Recombinant E.coli pykF protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-470aa
• Description: pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Molecular Mass: 53.3kDa (494aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENKGVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTDRVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: pykF pyruvate kinase I [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: pykF
• Synonyms: pykF; ECK1672; JW1666; pyruvate kinase I; pyruvate kinase I (formerly F), fructose stimulated
• Gene ID: 946179
• Protein Refseq: NP_416191
• Pathway: Biosynthesis of secondary metabolites, organism-specific biosystem; ChREBP activates metabolic gene expression, organism-specific biosystem; Glucose metabolism, organism-specific biosystem