Recombinant E.coli NfsB, His-tagged

• Cat.No.: nfsB-2767E
• Product Overview: Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 26.0 kDa (237aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTL LQY SPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVF CAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHD DAE WMA KQV YLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYT SLVVV P VGHHSVEDFN ATLPKSRLPQ NITLTEV.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Trinitrotoluene degradation

Gene Information

• Gene Name: nfsB dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive; dprA; ECK0570; JW0567; nfnB; nfsI; ntr; oxygen-insensitive NAD(P)H nitroreductase
• Gene ID: 945778
• Protein Refseq: NP_415110