Recombinant E.coli MsrB, His-tagged

• Cat.No.: msrB-980E
• Product Overview: Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-137aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol; 0.1M NaCl,1mM DTT
• Molecular Weight: 17.6 kDa (157aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFYEPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: msrB methionine sulfoxide reductase B [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: msrB; methionine sulfoxide reductase B; ECK1776; JW1767; EC 1.8.4.12
• Gene ID: 947188
• Protein Refseq: NP_416292